NADPH Cytochrome P450 Reductase

NADPH P450 reductase, PDB entry 1AMO

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About

The NADPH-dependent cytochrome P450 reductase (CPR) is a membrane bound protein localized in the ER membrane. CPR is a multidomain protein with three cofactor binding domains (FMN, FAD and NADPH) and a linker domain situated between the FMN and FAD/NADPH domains. CPR donates electrons from the two-electron donor NADPH to the heme of P450 in a coupled two-step reaction as illustrated in picture below. NADPH binds to the NADPH-binding domain and electrons are shuttled from NADPH through FAD and FMN to the P450 heme. Please see the CPR structure below, color correlated with the reaction mechanism to its right.

NADPH P450 Reductase structure (R. norvegicus) and reaction mechanism courtesy of Kenneth Jensen

(Click for a larger image.)

Publications

Plant NADPH-cytochrome P450 oxidoreductases, Jensen K, Møller BL. Phytochemistry [Epub ahead of print]

CPR Sequences

Table Key

Chlamydomonas

Selaginella

Mosses

Gymnosperms

Monocotyledons

Eudicotyledons

CreCPR	SmCPRa	SmCPRb	SmCPRc	PpCPRa
PpCPRb	PpCPRc	PpCPRd	TchCPR	TcuCPR
PmCPR	OsCPR2a	OsCPR2b	OsCPR2c	SbCPR2c
SbCPR2a	SbCPR2b	TaCPR2c	TaCPR2a	ZmCPR2c
ZmCPR2b1	ZmCPR2b2	AmCPR2	AtATR1	AtATR2
AaCPR2	CaCPR1	CroCPR2	CeCPR1	CbCPR2
EcCPR2	GmCPR1	GhCPR2	GhCPR1	HtCPR2a
HtCPR2b	HaCPR1	LjCPR2	OpCPR1	PsoCPR1
PcCPR2a	PcCPR2b	PhCPR1	PhCPR2	PsaCPR2
PtCPR2b	PtCPR2a	PtCPR1	RcCPR1	RcCPR2
SrCPR2	VsCPR1	VrCPR1	VvCPR2

Cre: Chlamydomonas reinhardtii	Sm: Selaginella moellendorffii	Pp: Physcomitrella patens	Tch: Taxus chinensis	Tcu: Taxus cuspidata	Pm: Pseudotsuga menziesii
Os: Oryza sativa	Sb: Sorghum bicolor	Ta: Triticum aestivum	Zm: Zea mays	Am: Ammi majus	At: Arabidopsis thaliana
Aa: Artemisia annua	Ca: Capsicum annuum	Cro: Catharanthus roseus	Ce: Centaurium erythraea	Cb: Coleus blumei	Ec: Eschscholzia californica
Gm: Glycine max	Gh: Gossypium hirsutum	Ht: Helianthus tuberosus	Ha: Hypericum androsaemum	Lj: Lotus japonicus	Op: Ophiorrhiza pumila
Pso: Papaver somniferum	Pc: Petroselinum crispum	Ph: Petunia hybrida	Psa: Pisum sativum	Pt: Populus trichocarpa x Populus deltoides	Rc: Ricinus communis
Sr: Stevia rebaudiana	Vs: Vicia sativa	Vr: Vigna radiata	Vv: Vitis vinifera

Alignments

This multiple alignment was made with ClustalX 2.0.11 for Windows.

An alignment

Phylogenetic Trees

This phylogenetic tree was made using MEGA 4 for Windows.

A Phylogenetic tree

alignment

Sequence Logos

Logos were made using weblogo.berkeley.edu.

A logo

alignment

Chromosome Maps

chromosome map

Arabidopsis

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